International Journal of Systematic and Evolutionary Microbiology 63: 418-422

Streptococcus troglodytae sp. nov., from the Chimpanzee Oral Cavity

Masaaki Okamoto, Susumi Imai, Mayu Miyanohara, Wataru Saito, Yasuko Momoi, Tomoko Abo, Yoshiaki Nomura, Tomoko Ikawa, Takumi Ogawa, Takako Miyabe-Nishiwaki, Akihisa Kaneko, Akino Watanabe, Shohei Watanabe, Misato Hayashi, Masaki Tomonaga, Nobuhiro Hanada

Abstract

Six strains, TKU 25, TKU 28, TKU 30, TKU 31T, TKU 33 and TKU 34 were isolated from the chimpanzee (Pan troglodytes) oral cavity. The colonies of strains grown on the Mitis-Salivarius agar were similar in morphology to that of Streptococcus mutans. The novel strains were Gram stain positive, facultative anaerobic cocci that lacked catalase activity. Analysis of their partial 16S rRNA gene sequences of these isolates showed that the most closely related species was S. mutans (96.4 %). The next closely related species to the isolates were Streptococcus devriesei (94.5 %) and Streptococcus downei (93.9 %). These isolates could be distinguished from S. mutans by the inulin fermentation and alkaline phosphatase activity (API-ZYM system). The peptideglycan type was Glu-Lys-Ala3. Strains were not susceptible to bacitracin. On the basis of the phenotypic characterization, partial 16S rRNA gene and two housekeeping gene (groEL and sodA) sequences data, we propose a novel taxa, Streptococcus troglodytae for TKU 31T (type strain =JCM 18038T =DSM 25324T) for this newly described isolate.

Okamoto M, Imai S, Miyanohara M, Saito W, Momoi Y, Abo T, Nomura Y, Ikawa T, Ogawa T, Miyabe-Nishiwaki T, Kaneko A, Watanabe A, Watanabe S, Hayashi M, Tomonaga M, Hanada N (2012) Streptococcus troglodytae sp. nov., from the Chimpanzee Oral Cavity International Journal of Systematic and Evolutionary Microbiology 63: 418-422 , doi: 10.1099/ijs.0.039388-0